msi small molecule inhibitor ro 08 2750 Search Results


ro 08-2750  (Bio-Techne corporation)
94
Bio-Techne corporation ro 08-2750
Ro 08 2750, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msi+small+molecule+inhibitor+ro+08+2750/Ro+08-2750/custom%402272%4036966175
Average 94 stars, based on 1 article reviews
ro 08-2750 - by Bioz Stars, 2026-09
94/100 stars
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binding  (MedChemExpress)
94
MedChemExpress binding
Binding, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msi+small+molecule+inhibitor+ro+08+2750/Ro+08-2750/ppr0354143-63-1-5
Average 94 stars, based on 1 article reviews
binding - by Bioz Stars, 2026-09
94/100 stars
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ro 08–2750 (ro; ngf inhibitor  (GlpBio Technology Inc)
90
GlpBio Technology Inc ro 08–2750 (ro; ngf inhibitor
The effect of double targeting of <t>NGF</t> and autophagy on SCs. A . CCK-8 proliferation assays of RSC96 cells treated with the control, RO <t>08–2750</t> (RO) (1 μM), chloroquine (CQ) (40 μM) or RO + CQ (1 μM + 40 μM). The OD 450 nm value was determined at 0 h, 24 h, 48 h, and 72 h (* p < 0.05). B . Transwell migration assays of RSC96 cells treated with the control, RO, CQ or RO + CQ.RSC96 were seeded in the upper chamber with 1% serum and the indicated drugs. The lower chamber were 10% serum culture medium. C . Statistics of migrated RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4B) (* p < 0.05). D . Autophagic flux detection of RSC96 treated with the control, RO, CQ or RO + CQ at 24 h. LC3 labeled with GFP (green) and RFP (red) and merged as yellow. Red arrow: autophagolysosomes. Yellow arrow: autophagosome. RO treatment could reduce both autophagolysosomes and autophagosomes. CQ treatment could increase both autophagolysosomes and autophagosomes. CQ treatment could also increase the ratio of autophagosomes to autophagolysosomes. RO + CQ treatment could increase the ratio of autophagosomes to autophagolysosomes compared with control. RO + CQ treatment could reduce autophagolysosomes and autophagosomes compared with single CQ group. E . Statistics of red and yellow puncta number in RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4D) (* p < 0.05). F . TEM image of RSC96 cells treated with RO, CQ or RO + CQ at 24 h. Autophagy and apoptosis signs were detected. Green arrow: autophagosome. Red arrow: apoptosis sign. Yellow arrow: dysfunctional lysosome (CQ inhibits the lysosome function and the dysfunctional lysosomes are condensed and shows high density in TEM image). G . Statistics of autophagosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). H . Statistics of dysfunctional lysosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). I . Statistics of cleaved PARP1-positive and cleaved PARP1-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). J . Statistics of cleaved caspase-3-positive and cleaved caspase-3-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). K . Western blotting of RSC96 cells treated with RO, CQ or RO + CQ. GAPDH was used as a loading control. RO, CQ or RO + CQ could induce the expression of cleaved PARP-1. RO, CQ or RO + CQ could inhibit ATG7 expression in RSC96 while could not influence ATG5. RO could inhibit LC3II formation while CQ or RO + CQ could promote LC3II accumulation
Ro 08–2750 (Ro; Ngf Inhibitor, supplied by GlpBio Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msi+small+molecule+inhibitor+ro+08+2750/ro+08+2750++ro++ngf+inhibitor/pmc08809009-35-16-21
Average 90 stars, based on 1 article reviews
ro 08–2750 (ro; ngf inhibitor - by Bioz Stars, 2026-09
90/100 stars
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cells  (Tocris)
93
Tocris cells
The effect of double targeting of <t>NGF</t> and autophagy on SCs. A . CCK-8 proliferation assays of RSC96 cells treated with the control, RO <t>08–2750</t> (RO) (1 μM), chloroquine (CQ) (40 μM) or RO + CQ (1 μM + 40 μM). The OD 450 nm value was determined at 0 h, 24 h, 48 h, and 72 h (* p < 0.05). B . Transwell migration assays of RSC96 cells treated with the control, RO, CQ or RO + CQ.RSC96 were seeded in the upper chamber with 1% serum and the indicated drugs. The lower chamber were 10% serum culture medium. C . Statistics of migrated RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4B) (* p < 0.05). D . Autophagic flux detection of RSC96 treated with the control, RO, CQ or RO + CQ at 24 h. LC3 labeled with GFP (green) and RFP (red) and merged as yellow. Red arrow: autophagolysosomes. Yellow arrow: autophagosome. RO treatment could reduce both autophagolysosomes and autophagosomes. CQ treatment could increase both autophagolysosomes and autophagosomes. CQ treatment could also increase the ratio of autophagosomes to autophagolysosomes. RO + CQ treatment could increase the ratio of autophagosomes to autophagolysosomes compared with control. RO + CQ treatment could reduce autophagolysosomes and autophagosomes compared with single CQ group. E . Statistics of red and yellow puncta number in RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4D) (* p < 0.05). F . TEM image of RSC96 cells treated with RO, CQ or RO + CQ at 24 h. Autophagy and apoptosis signs were detected. Green arrow: autophagosome. Red arrow: apoptosis sign. Yellow arrow: dysfunctional lysosome (CQ inhibits the lysosome function and the dysfunctional lysosomes are condensed and shows high density in TEM image). G . Statistics of autophagosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). H . Statistics of dysfunctional lysosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). I . Statistics of cleaved PARP1-positive and cleaved PARP1-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). J . Statistics of cleaved caspase-3-positive and cleaved caspase-3-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). K . Western blotting of RSC96 cells treated with RO, CQ or RO + CQ. GAPDH was used as a loading control. RO, CQ or RO + CQ could induce the expression of cleaved PARP-1. RO, CQ or RO + CQ could inhibit ATG7 expression in RSC96 while could not influence ATG5. RO could inhibit LC3II formation while CQ or RO + CQ could promote LC3II accumulation
Cells, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msi+small+molecule+inhibitor+ro+08+2750/Ro+08-2750/pmc08552705-59-1-14
Average 93 stars, based on 1 article reviews
cells - by Bioz Stars, 2026-09
93/100 stars
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ro 08 2750  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology ro 08 2750
The effect of double targeting of <t>NGF</t> and autophagy on SCs. A . CCK-8 proliferation assays of RSC96 cells treated with the control, RO <t>08–2750</t> (RO) (1 μM), chloroquine (CQ) (40 μM) or RO + CQ (1 μM + 40 μM). The OD 450 nm value was determined at 0 h, 24 h, 48 h, and 72 h (* p < 0.05). B . Transwell migration assays of RSC96 cells treated with the control, RO, CQ or RO + CQ.RSC96 were seeded in the upper chamber with 1% serum and the indicated drugs. The lower chamber were 10% serum culture medium. C . Statistics of migrated RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4B) (* p < 0.05). D . Autophagic flux detection of RSC96 treated with the control, RO, CQ or RO + CQ at 24 h. LC3 labeled with GFP (green) and RFP (red) and merged as yellow. Red arrow: autophagolysosomes. Yellow arrow: autophagosome. RO treatment could reduce both autophagolysosomes and autophagosomes. CQ treatment could increase both autophagolysosomes and autophagosomes. CQ treatment could also increase the ratio of autophagosomes to autophagolysosomes. RO + CQ treatment could increase the ratio of autophagosomes to autophagolysosomes compared with control. RO + CQ treatment could reduce autophagolysosomes and autophagosomes compared with single CQ group. E . Statistics of red and yellow puncta number in RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4D) (* p < 0.05). F . TEM image of RSC96 cells treated with RO, CQ or RO + CQ at 24 h. Autophagy and apoptosis signs were detected. Green arrow: autophagosome. Red arrow: apoptosis sign. Yellow arrow: dysfunctional lysosome (CQ inhibits the lysosome function and the dysfunctional lysosomes are condensed and shows high density in TEM image). G . Statistics of autophagosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). H . Statistics of dysfunctional lysosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). I . Statistics of cleaved PARP1-positive and cleaved PARP1-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). J . Statistics of cleaved caspase-3-positive and cleaved caspase-3-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). K . Western blotting of RSC96 cells treated with RO, CQ or RO + CQ. GAPDH was used as a loading control. RO, CQ or RO + CQ could induce the expression of cleaved PARP-1. RO, CQ or RO + CQ could inhibit ATG7 expression in RSC96 while could not influence ATG5. RO could inhibit LC3II formation while CQ or RO + CQ could promote LC3II accumulation
Ro 08 2750, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msi+small+molecule+inhibitor+ro+08+2750/Ro+08-2750/pm30092219-31-38-42
Average 90 stars, based on 1 article reviews
ro 08 2750 - by Bioz Stars, 2026-09
90/100 stars
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ro 08-2750 b6996  (ApexBio)
90
ApexBio ro 08-2750 b6996
The effect of double targeting of <t>NGF</t> and autophagy on SCs. A . CCK-8 proliferation assays of RSC96 cells treated with the control, RO <t>08–2750</t> (RO) (1 μM), chloroquine (CQ) (40 μM) or RO + CQ (1 μM + 40 μM). The OD 450 nm value was determined at 0 h, 24 h, 48 h, and 72 h (* p < 0.05). B . Transwell migration assays of RSC96 cells treated with the control, RO, CQ or RO + CQ.RSC96 were seeded in the upper chamber with 1% serum and the indicated drugs. The lower chamber were 10% serum culture medium. C . Statistics of migrated RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4B) (* p < 0.05). D . Autophagic flux detection of RSC96 treated with the control, RO, CQ or RO + CQ at 24 h. LC3 labeled with GFP (green) and RFP (red) and merged as yellow. Red arrow: autophagolysosomes. Yellow arrow: autophagosome. RO treatment could reduce both autophagolysosomes and autophagosomes. CQ treatment could increase both autophagolysosomes and autophagosomes. CQ treatment could also increase the ratio of autophagosomes to autophagolysosomes. RO + CQ treatment could increase the ratio of autophagosomes to autophagolysosomes compared with control. RO + CQ treatment could reduce autophagolysosomes and autophagosomes compared with single CQ group. E . Statistics of red and yellow puncta number in RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4D) (* p < 0.05). F . TEM image of RSC96 cells treated with RO, CQ or RO + CQ at 24 h. Autophagy and apoptosis signs were detected. Green arrow: autophagosome. Red arrow: apoptosis sign. Yellow arrow: dysfunctional lysosome (CQ inhibits the lysosome function and the dysfunctional lysosomes are condensed and shows high density in TEM image). G . Statistics of autophagosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). H . Statistics of dysfunctional lysosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). I . Statistics of cleaved PARP1-positive and cleaved PARP1-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). J . Statistics of cleaved caspase-3-positive and cleaved caspase-3-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). K . Western blotting of RSC96 cells treated with RO, CQ or RO + CQ. GAPDH was used as a loading control. RO, CQ or RO + CQ could induce the expression of cleaved PARP-1. RO, CQ or RO + CQ could inhibit ATG7 expression in RSC96 while could not influence ATG5. RO could inhibit LC3II formation while CQ or RO + CQ could promote LC3II accumulation
Ro 08 2750 B6996, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msi+small+molecule+inhibitor+ro+08+2750/ro+08+2750+b6996/pmc08830500-45-3-9
Average 90 stars, based on 1 article reviews
ro 08-2750 b6996 - by Bioz Stars, 2026-09
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ppads  (Alomone Labs)
90
Alomone Labs ppads
The effect of double targeting of <t>NGF</t> and autophagy on SCs. A . CCK-8 proliferation assays of RSC96 cells treated with the control, RO <t>08–2750</t> (RO) (1 μM), chloroquine (CQ) (40 μM) or RO + CQ (1 μM + 40 μM). The OD 450 nm value was determined at 0 h, 24 h, 48 h, and 72 h (* p < 0.05). B . Transwell migration assays of RSC96 cells treated with the control, RO, CQ or RO + CQ.RSC96 were seeded in the upper chamber with 1% serum and the indicated drugs. The lower chamber were 10% serum culture medium. C . Statistics of migrated RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4B) (* p < 0.05). D . Autophagic flux detection of RSC96 treated with the control, RO, CQ or RO + CQ at 24 h. LC3 labeled with GFP (green) and RFP (red) and merged as yellow. Red arrow: autophagolysosomes. Yellow arrow: autophagosome. RO treatment could reduce both autophagolysosomes and autophagosomes. CQ treatment could increase both autophagolysosomes and autophagosomes. CQ treatment could also increase the ratio of autophagosomes to autophagolysosomes. RO + CQ treatment could increase the ratio of autophagosomes to autophagolysosomes compared with control. RO + CQ treatment could reduce autophagolysosomes and autophagosomes compared with single CQ group. E . Statistics of red and yellow puncta number in RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4D) (* p < 0.05). F . TEM image of RSC96 cells treated with RO, CQ or RO + CQ at 24 h. Autophagy and apoptosis signs were detected. Green arrow: autophagosome. Red arrow: apoptosis sign. Yellow arrow: dysfunctional lysosome (CQ inhibits the lysosome function and the dysfunctional lysosomes are condensed and shows high density in TEM image). G . Statistics of autophagosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). H . Statistics of dysfunctional lysosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). I . Statistics of cleaved PARP1-positive and cleaved PARP1-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). J . Statistics of cleaved caspase-3-positive and cleaved caspase-3-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). K . Western blotting of RSC96 cells treated with RO, CQ or RO + CQ. GAPDH was used as a loading control. RO, CQ or RO + CQ could induce the expression of cleaved PARP-1. RO, CQ or RO + CQ could inhibit ATG7 expression in RSC96 while could not influence ATG5. RO could inhibit LC3II formation while CQ or RO + CQ could promote LC3II accumulation
Ppads, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/msi+small+molecule+inhibitor+ro+08+2750/GNF+5837/pm29061636-230-10-40
Average 90 stars, based on 1 article reviews
ppads - by Bioz Stars, 2026-09
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ro 08-2750  (BOC Sciences)
N/A
Ro 08-2750 is a potent and selective Nerve growth factor (NGF) inhibitor that binds the NGF dimer (KD ~ 1 μM). NGF has potential effects on matrix turnover activity and influences the catabolic/anabolic balance of
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rabbit anti- gpr108 polyclonal antibody  (Cusabio)
N/A
Rabbit anti-Human GPR108 Polyclonal Antibody
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ro 08-2750  (Toronto Research Chemicals)
N/A
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s100a4 antibody (s100a4/2750r) [pe]  (Novus Biologicals)
N/A
The S100A4 Antibody (S100A4/2750R) [PE] from Novus is a S100A4 antibody to S100A4. This antibody reacts with Human, Mouse. The S100A4 antibody has been validated for the following applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/
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compound e, gamma;-secretase inhibitor  (Aladdin Scientific Corporation)
N/A
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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Image Search Results


The effect of double targeting of NGF and autophagy on SCs. A . CCK-8 proliferation assays of RSC96 cells treated with the control, RO 08–2750 (RO) (1 μM), chloroquine (CQ) (40 μM) or RO + CQ (1 μM + 40 μM). The OD 450 nm value was determined at 0 h, 24 h, 48 h, and 72 h (* p < 0.05). B . Transwell migration assays of RSC96 cells treated with the control, RO, CQ or RO + CQ.RSC96 were seeded in the upper chamber with 1% serum and the indicated drugs. The lower chamber were 10% serum culture medium. C . Statistics of migrated RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4B) (* p < 0.05). D . Autophagic flux detection of RSC96 treated with the control, RO, CQ or RO + CQ at 24 h. LC3 labeled with GFP (green) and RFP (red) and merged as yellow. Red arrow: autophagolysosomes. Yellow arrow: autophagosome. RO treatment could reduce both autophagolysosomes and autophagosomes. CQ treatment could increase both autophagolysosomes and autophagosomes. CQ treatment could also increase the ratio of autophagosomes to autophagolysosomes. RO + CQ treatment could increase the ratio of autophagosomes to autophagolysosomes compared with control. RO + CQ treatment could reduce autophagolysosomes and autophagosomes compared with single CQ group. E . Statistics of red and yellow puncta number in RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4D) (* p < 0.05). F . TEM image of RSC96 cells treated with RO, CQ or RO + CQ at 24 h. Autophagy and apoptosis signs were detected. Green arrow: autophagosome. Red arrow: apoptosis sign. Yellow arrow: dysfunctional lysosome (CQ inhibits the lysosome function and the dysfunctional lysosomes are condensed and shows high density in TEM image). G . Statistics of autophagosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). H . Statistics of dysfunctional lysosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). I . Statistics of cleaved PARP1-positive and cleaved PARP1-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). J . Statistics of cleaved caspase-3-positive and cleaved caspase-3-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). K . Western blotting of RSC96 cells treated with RO, CQ or RO + CQ. GAPDH was used as a loading control. RO, CQ or RO + CQ could induce the expression of cleaved PARP-1. RO, CQ or RO + CQ could inhibit ATG7 expression in RSC96 while could not influence ATG5. RO could inhibit LC3II formation while CQ or RO + CQ could promote LC3II accumulation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Autophagic Schwann cells promote perineural invasion mediated by the NGF/ATG7 paracrine pathway in pancreatic cancer

doi: 10.1186/s13046-021-02198-w

Figure Lengend Snippet: The effect of double targeting of NGF and autophagy on SCs. A . CCK-8 proliferation assays of RSC96 cells treated with the control, RO 08–2750 (RO) (1 μM), chloroquine (CQ) (40 μM) or RO + CQ (1 μM + 40 μM). The OD 450 nm value was determined at 0 h, 24 h, 48 h, and 72 h (* p < 0.05). B . Transwell migration assays of RSC96 cells treated with the control, RO, CQ or RO + CQ.RSC96 were seeded in the upper chamber with 1% serum and the indicated drugs. The lower chamber were 10% serum culture medium. C . Statistics of migrated RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4B) (* p < 0.05). D . Autophagic flux detection of RSC96 treated with the control, RO, CQ or RO + CQ at 24 h. LC3 labeled with GFP (green) and RFP (red) and merged as yellow. Red arrow: autophagolysosomes. Yellow arrow: autophagosome. RO treatment could reduce both autophagolysosomes and autophagosomes. CQ treatment could increase both autophagolysosomes and autophagosomes. CQ treatment could also increase the ratio of autophagosomes to autophagolysosomes. RO + CQ treatment could increase the ratio of autophagosomes to autophagolysosomes compared with control. RO + CQ treatment could reduce autophagolysosomes and autophagosomes compared with single CQ group. E . Statistics of red and yellow puncta number in RSC96 cells treated with the control, RO, CQ or RO + CQ (cell image: Fig. 4D) (* p < 0.05). F . TEM image of RSC96 cells treated with RO, CQ or RO + CQ at 24 h. Autophagy and apoptosis signs were detected. Green arrow: autophagosome. Red arrow: apoptosis sign. Yellow arrow: dysfunctional lysosome (CQ inhibits the lysosome function and the dysfunctional lysosomes are condensed and shows high density in TEM image). G . Statistics of autophagosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). H . Statistics of dysfunctional lysosome numbers of RSC96 cells treated with RO, CQ or RO + CQ (cell image: Fig. 4F) (* p < 0.05). I . Statistics of cleaved PARP1-positive and cleaved PARP1-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). J . Statistics of cleaved caspase-3-positive and cleaved caspase-3-negative RSC96 cell numbers treated with the control, RO, CQ or RO + CQ (cell image: Fig. D) (* p < 0.05). K . Western blotting of RSC96 cells treated with RO, CQ or RO + CQ. GAPDH was used as a loading control. RO, CQ or RO + CQ could induce the expression of cleaved PARP-1. RO, CQ or RO + CQ could inhibit ATG7 expression in RSC96 while could not influence ATG5. RO could inhibit LC3II formation while CQ or RO + CQ could promote LC3II accumulation

Article Snippet: Cytokines, antibodies and reagents were purchased from the indicated suppliers: nerve growth factor (NGF; Peprotech, 450–01), RO 08–2750 (RO; NGF inhibitor, GLPBIO, Cas No. 37854–59-4), chloroquine diphosphate salt (CQ; lysosomal inhibitor, Sigma–Aldrich, C6628), hematoxylin and eosin (HE, Beyotime Biotechnology, C0105), and 4′,6-diamidino-2-phenylindole (DAPI, Beyotime Biotechnology, C1002).

Techniques: CCK-8 Assay, Control, Migration, Labeling, Western Blot, Expressing